Fig. 5.

Proteomics of the ANL labeled proteins that are derived from muscle of old C57BL/6 parabiotic partners of young MetRS^L274G mice. a Scheme of the application of antibody arrays to BONCAT focused proteomics. b Representative images of scanned antibody arrays that were performed with the muscle lysates from old C57BL/6 animals parabiosed to young MetRS^L274G partners as compared to isogenic C57BL/6 pairs. The lysates were isolated from CTX injured TA and Gastroc muscles at 3 days post injury. c For these antibody arrays a Click-western blotting was performed to confirm detectable ANL-labeling in the muscle lysates from old C57BL/6 to young MetRS^L274G parabionts, as compared to the background noise of the syngeneic C57BL/6 parabionts. Coomassie blue staining demonstrates equal protein loading. d Heat Map of the proteins that were detected at >2.0-fold higher levels in the muscle of old C57BL/6 mice parabiosed to young MetRS^L274G, as compared to those parabiosed to young C57BL/6 animals with statistical significance of P < 0.05 (Wilcoxon rank sum test). n = 3. Color-coding: myogenic regulators (blue), inflammation regulators (pink), tissue remodeling proteins (green). e Schematic cartoon of the possible roles of the young proteins (colored boxes that correspond to protein grouping in the Heat Map) that were detected in the muscle of old parabiotic partners, grouped by canonical roles in regulating myogenic proliferation and differentiation, TGF-beta/myostatin, Notch and WNT signaling pathways, tissue remodeling and inflammation. Question marks signify putative roles