Fig. 2.

MYB3R3 accumulation in the root tip. a, c Confocal microscopy images of root tips. Five-day-old myb3r3 seedlings harbouring ProMYB3R3::MYB3R3-GFP were treated with 2 μM zeocin for the indicated times a, or with 2 μM zeocin, 50 μM MG132 or 25 μM roscovitine for 24 h c. Scale bars, 100 μm. b, d Protein level of MYB3R3-GFP in roots. Ten-day-old myb3r3 seedlings harbouring ProMYB3R3::MYB3R3-GFP were treated with 5 or 10 μM zeocin for 24 or 48 h b, or with 2 μM zeocin, 50 μM MG132 or 25 μM roscovitine for 24 h d. Forty ( b ) or 20 µg ( d ) of total protein extracted from roots were subjected to immunoblotting using antibodies against GFP, α-tubulin or histone H3. Protein extract from the myb3r3 mutant was used as a control ( d ). Relative levels of MYB3R3-GFP are expressed as the fold change, normalized with respect to the band of α-tubulin or histone H3