Fig. 3.

Changes in nuclear area upon DNA damage. a Assignment of root meristematic cells to distinct cell cycle stages. In the Cytrap marker system, RFP and GFP fluorescence represent cells in S-to-G2 and late G2-to-M (metaphase), respectively. EdU staining of the whole nucleus or speckled signals indicate cells in early S or late S, respectively. Representative patterns of EdU and Cytrap signals are shown for early S, late S, late G2 and G1. Scale bar, 10 μm. b Nuclear area at distinct cell cycle stages. By using 5-day-old seedlings, cell cycle stages of cells in the root meristem were identified based on Cytrap and EdU signals as shown in a. Dotted lines indicate the average nuclear area (n > 68). c Nuclear area of root meristematic cells after treatment with zeocin. Five-day-old WT seedlings were transferred to medium with 2 µM zeocin and grown for 24 or 72 h. Dotted lines indicate the average nuclear area (n > 84). d Nuclear area in the myb3r3 mutant harbouring ProMYB3R3::MYB3R3-GFP or ProMYB3R3::MYB3R3AAA-GFP. Five-day-old seedlings were transferred to medium with 2 µM zeocin and grown for 24 or 72 h. Nuclear area of GFP-expressing cells in the root meristem was measured. Dotted lines indicate the average nuclear area (n > 118)