Fig. 7.

DNA damage promotes the binding of MYB3R3 to KNOLLE and CYCB1;2 promoters. a ChIP-PCR analysis of G2/M-specific genes. Chromatin bound to MYB3R3 was collected by immunoprecipitation with (+Ab) or without (−Ab) anti-GFP antibodies using roots of 10-day-old myb3r3 plants carrying ProMYB3R3::MYB3R3-GFP. Fold enrichment of each promoter region was determined by normalizing the recovery rate against that of samples immunoprecipitated without the antibody. CDKA;1 was used as a control that is expressed throughout the cell cycle. Data are presented as mean ± SD of three biological replicates. Significant differences from the control immunoprecipitated without the antibody were determined by Student’s t-test: ***P < 0.001. b, c ChIP-PCR analysis using zeocin-treated roots. Ten-day-old myb3r3 seedlings carrying ProMYB3R3::MYB3R3-GFP were treated with or without 2 μM zeocin for 24 h (b) or for the indicated times (c), and used for ChIP assay. Fold enrichment of each promoter region was determined by normalizing the recovery rate against that of samples without zeocin treatment. Data are presented as mean ± SD of three biological replicates. Significant differences from the control without zeocin treatment were determined by Student’s t-test: ***P < 0.001 ( b )