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. 2017 Sep 21;8:635. doi: 10.1038/s41467-017-00676-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — Quantitative RT-PCR analysis of G2/M-specific genes. Six-day-old seedlings were transferred to medium containing 2 µM zeocin and grown for the indicated times. Total RNA was isolated from the region 1 cm from the root tip. The expression levels were normalized to that of ACTIN2, and are indicated as relative values, with that for the time of transfer to zeocin-containing medium set to 1. Data are presented as mean ± SD of three biological replicates. Significant differences from WT were determined by Student’s t-test: *P < 0.05; **P < 0.01; ***P < 0.001