Fig. 1.

RAN effect on cell growth. a Experimental scheme for growth curve and viability determination. b C2C12 cells were seeded in 60 × 15 mm culture dishes at 20 % confluence and grown in GM medium with or without RAN, and DM medium. Medium was changed every 24 h and the experiment lasted until control cells achieved 40 % of confluence (3 days). As shown in panel b, RAN increases C2C12 proliferative potential without cytotoxic effects. c To confirm the results obtained by growth curve, we analyzed p53 protein content: Western blot analysis (panel c) shows the p53 significant increment in DM condition, confirming an inhibition of the cell cycle only in this condition, as reported in literature. In GM and GM-RAN cells p53 levels remain comparable with basal condition. d Experimental scheme for RAN treatment during proliferative, differentiation and post differentiation phases. Representative immunoblots of analyzed proteins are shown. Anova test: >p ≤ 0.05, t test: * p ≤ 0.05 vs. GM