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. 2016 Dec 8;58(1):33–45. doi: 10.1007/s12020-016-1181-5

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© The Author(s) 2016

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — RAN action on mitochondria in C2C12 myoblasts. We analyzed RAN action on mitochondria and ROS levels during the proliferation phase utilizing a specific staining procedure: a b, c MITO CytoPainter staining (40X): in all proliferative phases (a 24 h, b 48 h, c 72 h), RAN did not modify the number of mitochondria compared with control (GM and DM) as shown in quantification (e). d Cell Rox^® staining (20X): RAN appears to be able to decrease ROS synthesis in the basal state (f) and after H₂O₂-induced oxidative stress