Fig. 6.

RAN action during the differentiation process: morphological studies. a An important marker of muscle differentiation progression is the increase of MyHC positive cells. MyHC immunofluorescence (20X) indicated that in RAN-treated cells the number of MyHC-positive myotubes was significantly higher compared with control (24 h), as reported in the quantification graph. b During the proliferation phase we observed that RAN could influence CaMKII signaling. In the same manner, after 48 h of differentiation, RAN could improve pCaMKII protein expression compared with DM control (20X), as reported in the quantification. t test: *p ≤ 0.05 vs. DM