Figure 1.

Experimental timeline for drug treatments. Aβ 1-42 or vehicle was microinfused into cerebroventricle (0.4 μg, 1 μl, i.c.v.) 4 days after cannule implantation. BAY 60-7550 (0.3 and 3 mg/kg via i.p. (A); 5 and 10 μg, 2 μl via i.c.v. (B)) or vehicle was given once per day for 14 days. KT5823 (1 μl, 2 nmol), and H89 (1 μl, 5 nmol) were administered 30 minutes before treatment with BAY 60-7550. All the behavioral tests were performed 24 hours after last drug treatment. Behavioral tests were carried out on days 20–22, then the mice were sacrificed and biochemical assays were performed. MWM, Morris water maze; Sac, sacrifice; SD, Step-down passive avoidance test. N = 8–10 for each experiment.