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. 2017 Sep 21;7:12073. doi: 10.1038/s41598-017-12170-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Determination of inhibitor detection limit for the optimized Cruzipain assay. Different E-64 concentrations were assessed to determine the inhibitor concentration causing an average inhibition equivalent to the statistically robust cutoff ([I]^MINIMAL). For clarity, only E-64 concentrations ranging from 0 (enzyme control) to 4 nM are shown. To accurately describe the data dispersion, 24 replicates were used for each concentration point.