Table 1.
Statistics for the plates during primary screening.
| Plate 1 | Plate 2 | |||
|---|---|---|---|---|
| Mean | SD | Mean | SD | |
| Cruzipain Control (C+) (RFU/sec) | 893.31 | 23.35 | 210.84 | 22.29 |
| Negative Control (C−)(RFU/sec) | −2.68 | 1.44 | 0.80 | 0.13 |
| E-64 Control (RFU/sec) | 19.30 | 4.84 | 6.36 | 0.50 |
| Compounds (RFU/sec) | 794.91 | 135.38 | 191.89 | 46.74 |
| Compounds Inhibition (%) | 11.03 | 15.11 | 9.02 | 22.25 |
| Z factor | 0.917 | 0.679 | ||
| Statistically robust cutoff (RFU/sec) | 823.26 | 143.97 | ||
| Hit threshold i (RFU/sec) | 388.77 | 51.67 | ||
| Hit threshold ii (%) | 56.36 | 75.77 | ||
| Autofluorescence cutoff (RFU) | 972818.33 | 229605.97 | ||