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. 2017 Sep 21;7:12100. doi: 10.1038/s41598-017-12121-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunophenotype of unstimulated and IFN-γ stimulated AT-MSC. (a) Representative flow cytometry analysis of the commonly used markers for MSC (CD45 and CD31, both negative, and CD105, CD13, CD73, CD90), and the immune-markers HLA-I, HLA-II, and PD-L1. Isotype (white histograms), unstimulated AT-MSC (grey histograms) and IFN-γ AT-MSC (black histograms). (b) Percentage positive cells and (c) Mean fluorescence intensities (MFI) of the markers on unstimulated and IFN-γ stimulated AT-MSC. Data are presented as mean ± SD from 5 independent experiments. P values refer to the condition without IFN-γ. Unpaired t-test was used for statistical analysis.