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. 2017 Sep 21;7:12100. doi: 10.1038/s41598-017-12121-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of MP on apoptosis of monocyte subsets measured by Annexin V staining. Monocytes were cultured overnight with 3 ratios of MP or MPγ (1:10,000, 1:40,000 and 1:80,000). (a) Percentage of Annexin V positive CD14⁺CD16⁻ classical monocytes, and (b) percentage of Annexin V positive CD14⁺CD16⁺ pro-inflammatory monocytes. Data represent mean ± SD of 5 experiments using MP from 3 different donors. Two-way ANOVA was used for statistical analysis. P values (*p < 0.05) refer to the control without MP.