Fig. 6.

Readout of changes of H3K9me3 at mouse major satellite repeat sequences using BiAD sensor 4. a Representative fluorescence microscopy images demonstrating the co-localization of BiFC signal, DAPI staining, and H3K9me3 antibody staining. b Application of the BiAD sensor to detect changes in H3K9me3 levels after dox induced expression of SUV39H1 in Suv39DKO iMEF cells. A BiFC signal could only be observed when the sensor was transfected in cells expressing SUV39H1 (middle panel). No BiFC signal was detected in cells expressing the catalytically inactive H324L SUV39H1 mutant (bottom panel), c Quantification of the experiments representatively shown in panels a, b. Error bars represent s.e.m. for two biological replicates (for details cf. Supplementary Tables 4 and 6). Scale bar for all images is 10 µm. All cells were fixed 48 h after transfection and the imaging and display settings of the images shown b are identical