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. 2017 Sep 21;7:12087. doi: 10.1038/s41598-017-11168-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tissue-specific expression analysis and subcellular localization of AnnSp2 in N. benthamiana protoplasts. (A) Tissue profiling analysis of AnnSp2 in different organs (Stigma; Stamen; Bud; Flowers; Root; OL, old leaf; ML, mature leaf; YL, young leaf; Fruit) of wild tomato LA0716 (Solanum pennellii) using qRT-PCR. (B) AnnSp2 localized in the nucleus. The GFP fusion of AnnSp2 was co-expressed with NLS-RFP in N. benthamiana protoplasts for 12 h and the images were taken with a confocal microscope. NLS-RFP is a control for nuclear localization. Bar = 50 µm.