Fig. 3.

MiR-320 mimics influences the proliferation of U87 and U251. a Growth curves of U87 and U251 cells transfected with miR-320 or NC. b and c U87 and U251 cells transfected with miR-320 mimics were cultured for 2 weeks. Colonies were fixed with methanol and stained with 0.1% crystal violet. Visible colonies were manually counted. d Flow cytometric analysis of the indicated glioma cells transfected with NC or miR-320. e Cell cycle distribution of U87 and U251 cells transfected with miR-320 mimics or NC. *P < 0.05, **P < 0.01, ***P < 0.001, compared with negative control (at the same time point)