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. 2017 Jul 12;14(3):1993–1998. doi: 10.3892/etm.2017.4791

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Morpholino-ASO interventions on the SMA-01 urine cell line. (A) Cellular morphology of urine cells (A) prior to electroporation and (B) 24 h after electroporation. (C) Detection of SMN protein levels following 24 h treatment with ASO by western blotting. (D) SMN protein expression was quantified and expressed as the mean ± standard deviation percentage relative to actin (%): 0 pmol, 100%; 10 pmol, 151.0±10.0%; 20 pmol, 151.4±26.9%; 40 pmol, 194.6±22.3%. *P<0.05. Scale bars=100 µm. ASO, antisense oligo-treated; SMA, Spinal muscular atrophy; SMN, survival motor neuron 1.