Figure 2.

Effect of gemcitabine combined with GC7 on the sensitivity of pancreatic cancer cell lines. (A-C) Pancreatic cancer cell lines (Panc-1, BxPc3 and T3-M4) were incubated with different concentrations of GC7 over a period of 48 h. Cell Counting Kit-8 values of the treated pancreatic cancer cells were normalized to the control group with the absence of GC7. *P<0.05 vs. 0 µM GC7. (B) Western blot analysis was performed to determine eIF5A2 expression in pancreatic cancer cells treated with GC7. Relative protein expression was quantified by band density with GAPDH serving as an internal control. (D-F) Cell viability of pancreatic cancer cells after treatment with gemcitabine or gemcitabine combined with GC7. Cell viability was determined using the Cell Counting Kit-8 assay method. ^#P<0.05 vs. Gemcitabine.