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. 2017 Sep 22;8:1641. doi: 10.3389/fpls.2017.01641

FIGURE 3.

FIGURE 3

Seedling growth responses to low light conditions of the dlk2-3 mutant and its combinations with htl-3 and d14-1 mutants. (A) Seedling phenotypes of 5-day-old dlk2-3, d14-1 and htl-3 mutants and their combinations either untreated or exposed to 10 μM of (+)5DS and (–)5DS. Seeds were sown on 0.5XMS plates with 1% sucrose and supplemented with 10 μM of each compound as indicated. To initiate germination, seeds were dark stratified and were kept in red light (10 μmol m-2 s-1; LED) for 10 min. Plates were incubated for 5 d under low light conditions (7 μmol m-2 s-1; 21°C). (B,C) Hypocotyl elongation (B) and cotyledon expansion (C) responses of dlk2-3, d14-1 and htl-3 mutants and their combinations to 10 μM of (+)5DS and (–)5DS applications as compared to wild type Col-0 seedlings grown in low light for 5 days. Data are means of 3 independent experiments, >30 seedlings in each. Bars with the same letter are not significantly different from each other (mean ± SD; ANOVA, P < 0.01, Tukey’s HSD test).