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. 2017 Sep 22;8:1641. doi: 10.3389/fpls.2017.01641

FIGURE 4.

FIGURE 4

DLK2 overexpression (OE) lines exhibit elongated hypocotyl response to low light conditions. (A) Hypocotyl elongation responses of low light grown DLK2 OE (Ler) and DLK2 OE (dlk2-2) lines (10 days old). Seeds were germinated and grown on 0.5 × MS plates with 1% sucrose and supplemented with 1, 5, and 10 μM of rac-GR24 applications [DLK2 OE (Ler)] as compared to wild type seedlings. Data are means of 5 independent experiments, >30 seedlings in each. Asterisks represent a significant difference from Ler or Col-0 under the same treatment (mean ± SD; ANOVA, P < 0.025). Real-time PCR was corroborated in all lines with three biological replicates (n = 3, 15 10-day-old seedlings in each; Col-0 was set as calibrator); reactions were performed in quadruplicates. (B) Hypocotyl elongation responses of DLK2 OE (dlk2-3 d14-1 kai2-2) lines. Seeds were germinated and grown on 0.5 × MS plates with 1% sucrose and supplemented with 10 μM of (+)5DS and (–)5DS. Data are means of 3 independent experiments, >30 seedlings in each. Asterisks represent a significant difference from dlk2-3 d14-1 kai2-2 under the same treatment (mean ± SD; ANOVA, P < 0.025). Real-time PCR was corroborated in all lines with three biological replicates (n = 3, 15 10-day-old seedlings in each; Col-0 was set as calibrator); reactions were performed in quadruplicates. (C) Cotyledon expansion responses of DLK2 OE (dlk2-3 d14-1 kai2-2) lines. Seeds were germinated and grown on 0.5 × MS plates with 1% sucrose. Data are means of 3 independent experiments, >30 seedlings in each. Asterisks represent significant differences from dlk2-3 d14-1 kai2-2 under the same treatment (mean ± SD; ANOVA, P < 0.025).