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. 2017 Sep 20;8:1169. doi: 10.3389/fimmu.2017.01169

Figure 4.

Figure 4

Delayed CTLA4-Ig blocks the in vivo production of IFNγ by graft-infiltrating T cells. C57BL/6 mice were transplanted with BALB/c hearts and either remained untreated or given CLTA4-Ig 5 day posttransplantation. On day 6, recipients were injected with brefeldin A and 5 h later, mice were sacrificed, and IFNγ production by the graft-infiltrating T cells was determined by intracellular staining. (A) Cartoon depicting the experiment. (B) Sample IFNγ flow cytometry patterns from untreated (left column) or CLTA4-Ig treated (right column) heart transplant recipients. (C,D) Percentage of IFNγ-producing, (C) CD4+, and (D) CD8+ graft-infiltrating T cells in CTLA4-Ig treated recipients (HTx + CTLA4-Ig) was normalized and compared with the untreated group (HTx + No Rx). Data are presented from individual mice, N = 8 or 12 per group from at least three experiments, as mean ± SEM; ****p < 0.0001 by two-tailed t-test.