Skip to main content
. 2017 Sep 20;8:1169. doi: 10.3389/fimmu.2017.01169

Figure 7.

Figure 7

Delayed CTLA4-Ig moderately reduced the frequency of endogenous graft-specific T cells. (A) Sample gating strategies for the identification of 2W:I-Ab-specific CD4+ T cells or OVA-H-2Kb-specific CD8+ T cells. (B,C) C57BL/6 mice were transplanted with 2W-OVA.F1 hearts, without treatment (AR) or were treated with CLTA4-Ig on D6, 9, and 13 post-Tx (CTLA D6-14) prior to sacrifice on post-transplant day 14. Recipient spleens were analyzed for the total number of (B) 2W:I-Ab-specific CD4+ T cells; (C) OVA:H-2Kb-specific CD8+ T cells. (D,E) The percentage of CD44+CD62L− effector memory (EM), CD44+CD62L+ central memory (CM), and CD44−CD62L+ (Naïve) within the (D) 2W:I-Ab-specific CD4+ T cells or (E) OVA-H-2Kb-specific CD8+ T cells, with comparison to Naïve. Data shown from individual mice (N = 3–9/group) as mean ± SEM. *p < 0.05, ***p < 0.001, ****p < 0.0001 by one-way ANOVA. ####p < 0.0001 by two-tailed t-test.