Figure 4.

Pb18_Dld mRNA differential expression in different morphological forms of P. brasiliensis. (A–D) Morphological analysis by fluorescence microscopy. An aliquot of each fungal culture was incubated with Calcofluor white stain solution (50 μg/mL) for labeling the fungal cells. The bars correspond to 20 μm. (E) Evaluation of Pb18_Dld mRNA differential expression by RT-qPCR using cDNA from mycelia (A), mycelia-to-yeast (B), yeast (C), and yeast-to-mycelia (D). The β-actin and α-tubulin reference genes were used as endogenous controls to normalize the relative Pb18_Dld mRNA expression.