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Chromatographic separation steps. (A) AEX chromatography using Q-Sepharose as the stationary phase. (B) CEX chromatography on MonoS. Legend: (blue) UV absorbance at 280 nm (mAU), (orange) UV absorbance at 345 nm (mAU), (red) PPO activity (U/mL), (green) elution gradient (% buffer B), (brown) conductivity (mS/cm).
