Table 1. Purification of Polyphenol Oxidase from Apricot.
| purification stage | volume (mL) | total protein (mg) | total activitya (units) | specific activitya (units/mg protein) | purification (fold) | yield (%) |
|---|---|---|---|---|---|---|
| crude extract: 85% (NH4)2SO4 precipitation | 250 | 130 | 2570 | 19.8 | 1 | 100 |
| dialysis against Tris-HCl | 300 | 69.0 | 2312 | 33.5 | 1.7 | 89.0 |
| anion exchange: Q-Sepharose FF 20 mL column | 1.23 | 12.1 | 1506 | 125 | 6.3 | 58.6 |
| cation exchange: MonoS 5/50 GL column | 1.00 | 2.9 | 1311 | 459 | 23.2 | 51.0 |
a
Enzymatic activity was determined on 10 mM catechol in 50 mM citrate buffer, pH 4.5.