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. 2017 Jul 12;14(3):1947–1952. doi: 10.3892/etm.2017.4788

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Copyright: © Wu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — (A) Western blot analysis measured the effects of siRNA-mediated knockdown of TROP2. GAPDH was used as an internal control. Data are presented as the mean + standard deviation. Cells were transfected with (B) TROP2-homo-550 and (C) TROP2-homo-1100 siRNA and the viability was assessed using Cell counting kit-8 assay at five time points (24, 48, 72, 96 and 120 h). Transfected cells demonstrated a reduction in cell viability, and differences were significant when cells were cultured for 72 h (group 550) and 48 h (group 1100). Data are presented as the mean ± standard deviation. *P<0.05 vs. the control groups, ^#P<0.05 vs. group 550. TROP2, tumor-associated calcium signal transducer 2; siRNA, small interfering RNA; C, control; NC, negative control.