Figure 3.

Cordycepin inhibits TNF-α-induced phosphorylation of MAPKs and Akt in HA-VMSCs. HA-VMSCs were pretreated with various concentrations (1, 5 or 10 µM) of cordycepin for 2 h and subsequently incubated with TNF-α (10 ng/ml) for 30 min. (A) The expression of p-p38, p38, p-ERK1/2, ERK1/2, p-JNK, JNK, p-Akt and Akt proteins was detected via western blotting and representative blots were shown. (B-E) Quantification analysis was performed using BandScan 5.0 software. All data are presented as the mean ± standard error of the mean. All experiments were repeated a minimum of three times. ^&P<0.05 vs. the control group; *P<0.05 vs. the TNF-α group. TNF, tumor necrosis factor; MAPK, mitogen activated protein kinase; HA-VSMC, human aortic vascular smooth muscle cell; p, phosphorylated; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase; Akt, protein kinase B.