Figure 5.

Hep-2 cells were transfected with miR-138 mimics or co-transfected with miR-138 mimics and EZH2 expression plasmid. (A) Western blot analysis was performed to examine the protein expression of EZH2. (B) An MTT assay was used to examine cell proliferation. (C and D) Western blot analysis was performed to examine the protein levels of p-PI3K, t-PI3K, p-AKT and t-AKT. The relative activity of PI3K and AKT was determined. **P<0.01 vs. miR-138. miR, microRNA; p-PI3 K, phosphorylated phosphoinositide-3 kinase; t-AKT, total AKT; OD, optical density; EZH2, enhancer of zeste homologue 2.