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. Author manuscript; available in PMC: 2017 Sep 22.
Published in final edited form as: Neurobiol Dis. 2016 Mar 22;91:284–291. doi: 10.1016/j.nbd.2016.03.019

Fig. 2.

Fig. 2

MECP2 disrupts dendritic function and bidirectionally regulates Kibra levels in mouse brain. a,b. Representative traces of transfected primary cortical neurons from each replicate experiment (n = 5). c,d. Quantification of total dendritic length (TDL) and number of branches following mMECP2 overexpression (**p < 0.005 Tukey post-hoc following two-way ANOVA, F1,16 = 35.94, p < 0.0001). Error bars are mean ± SEM for replicate experiments. e. Representative Western blot and quantification of relative mKibra levels following AAV mediated overexpression of mMecp2 in primary cortical neurons (*p < 0.05, U = 4, Mann-Whitney U test, n = 6 independent assays per group). f,g. Representative Western blots of mKibra from hippocampus (hipp.), cerebellum (cb.), and cortex (ctx) of Mecp2−/y mice and Mecp2+/y controls (**p < 0.01, *p < 0.05 Tukey post-hoc following two-way ANOVA, F1,18 = 62.28, p < 0.0001, n = 4 per genotype). Scale bar represents 50 µm.