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. 2017 Jul 17;14(3):2625–2632. doi: 10.3892/etm.2017.4798

Figure 5.

Figure 5.

CLDN11 is a direct target of miR-421. (A) Luciferase activity assays involving transfection with luciferase vectors encoding a wild-type or mutant 3′UTR of CLDN11 were performed following co-transfection with miR-421 or NC mimic. Luciferase activity was normalized to that of the pMIR-REPORT™ β-gal plasmid. (B) Western blot analysis of CLDN11 protein expression in MKN28/MKN74 cells transfected with miR-421 or NC inhibitor. GAPDH served as an internal control. *P<0.05 vs. NC. CLDN11, claudin-11; miR, microRNA; NC, negative control.