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Effect of ulinastatin on HMGB1 levels in mouse myocardia. (A) Immunohistochemical analysis of HMGB1 protein expression in mice myocardia. Myocardial tissues were observed under a light microscope (scale bar, 20 µm). (B) Semi-quantification of HMGB1 staining. (C) Reverse transcription-quantitative polymerase chain reaction analysis of HMGB1 mRNA expression. *P<0.05 vs. control and #P<0.05 vs. DM. HMGB1, high mobility group box 1; DM, diabetes mellitus.
