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. 2017 Sep 22;8:719. doi: 10.3389/fphys.2017.00719

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Copyright © 2017 Srikakulapu, Upadhye, Rosenfeld, Marshall, McSkimming, Hickman, Mauldin, Ailawadi, Lopes, Taylor and McNamara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fat associated lymphoid clusters (FALCs) in human coronary artery PVAT. (A) Sections of diseased human coronary artery including PVAT (n = 1) were stained with hematoxylin and eosin. FALCs were identified in the PVAT adjacent to coronary artery. High magnification of FALCs (I and II) regions are indicated in dotted box. (B) Consecutive sections of hematoxylin stained slides were used for multiplex IHC staining (n = 1). CD20⁺ B cells, CD8⁺ T cells and PNAD⁺ high endothelial venules were observed in FALC. CD8⁺ T cells were marked with white arrows (merged lower left picture). DAPI stains for nuclear DNA.