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. 2017 Sep 22;8:719. doi: 10.3389/fphys.2017.00719

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Copyright © 2017 Srikakulapu, Upadhye, Rosenfeld, Marshall, McSkimming, Hickman, Mauldin, Ailawadi, Lopes, Taylor and McNamara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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B and T cells reside in human coronary artery PVAT. Coronary artery PVAT from human hearts (n = 6) explanted at the time of heart transplantation was analyzed by flow cytometry. (A) Gating strategy of B cells (CD20⁺), T cells (CD3⁺), and DN (CD45⁺ non B and T cells) in whole blood (WB), PVAT near LAD, and RCA for flow cytometry. Percentages of (B) B cells, (C) T cells, and (D) DN cells from total CD45⁺ cells were quantified in PVAT from the LAD and RCA, and WB as a comparator. Total number of (E) B cells and (F) T cells per gram adipose tissue was quantified. (G) Quantification of the percentage of B cells that were B-1 (CD20⁺ CD27⁺ CD43⁺). Results are mean ± SEM, unpaired student t-test was performed. Repeated measures one way ANOVA with Bonferroni's multiple comparison post-test was used to compare multiple groups (^*P < 0.05, ^**P < 0.01).