Composition of human SET1/MLL complexes in Hela cells based on immunoprecipitation experiments combined with mass spectrometry [adapted from (van Nuland et al. 2013)]. The SET1-2 (also termed KMT2F-G) and MLL1-4 (KMT2A-D) proteins are the catalytic subunits of six distinct human Set1-like complexes. These complexes contain four common subunits: ASH2L, DPY30, RBBP5 and WDR5, but also complex-specific components. Menin is exclusively found in MLL1 and MLL2-containing complexes. In MLL2 complexes in particular, menin has also been co-precipitated together with PSIP1.
Abbreviations: Absent, small, or homeotic-like (ASH2L), Bcl-2-related ovarian death gene (BOD), CXXC finger protein 1 (CFP1), host cell factor (HCF), lysine demethylase (KDM), lysine methyltransferase (KMT), mixed lineage leukemia (MLL), nuclear receptor coactivator (NCOA), O-Linked N-Acetylglucosamine transferase (OGT), PTIP associated protein (PA1), PC4 and SFRS1 interacting protein (PSIP1), Pax-interacting protein (PTIP), RB binding protein (RBBP), suvar enhancer-of-zeste, trithorax (SET), WD40 repeat (WDR)