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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: JACC Clin Electrophysiol. 2016 Dec 21;3(4):353–363. doi: 10.1016/j.jacep.2016.10.011

FIGURE 2. Radiofrequency Ablation of Epi Suppresses the Electrocardiographic and Arrhythmic Manifestations of Brugada Syndrome in Coronary-Perfused Canine Right Ventricular Wedge Model Generated Using a Combination of Pinacidil + Ajmaline.

FIGURE 2

Traces are as described in Figure 1. (A) Epi displays pronounced action potential notch at baseline. (B) Addition of pinacidil (2 μM) and ajmaline (3 μM) to the coronary perfusate induces the typical Brugada syndrome phenotype. The bipolar epicardial electrogram (Bip. Epi EG) shows fractionated electrogram activity and a late potential due to the development of concealed phase 2 re-entry. (C) Successful conduction of phase 2 re-entrant beat gives rise to ventricular tachycardia. (D) Recorded 40 min after Epi ablation and withdrawal of the provocative agents. Action potential recordings were obtained from midmyocardial (Mid) and subepicardial (Subepi) layers due to ablation of the epicardial cells. (E) Recorded 10 min after reintroduction of the provocative agents to the perfusate (in the same concentration as before). After Epi ablation, Brugada syndrome phenotype and arrhythmias were no longer inducible. Abbreviations as in Figure 1.