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. Author manuscript; available in PMC: 2017 Sep 22.
Published in final edited form as: Optica. 2017 Feb 20;4(2):263–271. doi: 10.1364/OPTICA.4.000263

Fig. 1.

Fig. 1

Optical principle and imaging performance of pLSFM. (A) Three axially and laterally staggered light-sheets (blue) illuminate different sections of a specimen. Fluorescence (green), is only generated within the beam waist of each light-sheet. (B) Image of staggered light-sheets in a solution of fluorescein. Scale bar 20 microns. (C) Simplified diagram of detection light path. Owing to their lateral and axial displacement, the fluorescence from each section can be independently detected in image space. (D) 3D rendering of volumetric pLSFM data before and (E) after stitching. (F) Lateral and axial point spread functions for the three cameras before and after 10 iterations of Richardson-Lucy deconvolution. Scale bar 1 μm. (G). Sagittal cross-section of cell shown in F. Scale bar 10 μm. (H-K) Cross-sections from G, shown normal to the coverslip. Scale bar 10 μm.