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. Author manuscript; available in PMC: 2017 Sep 22.
Published in final edited form as: Optica. 2017 Feb 20;4(2):263–271. doi: 10.1364/OPTICA.4.000263

Fig. 2.

Fig. 2

pLSFM enables sensitive time-lapse imaging. (A) 3D rendering of migrating MDA-MB-231 breast cancer cell as imaged by pLSFM, labeled with GFP-Tractin. (B) 3D rendering of same cell after 750 and (C) 1500 time points. (D) Wound healing response in collectively migrating and genome edited RPE cells, heterozygously expressing GFP-vimentin. Lateral (XY) maximum intensity projection. (E) Single plane cross-sections normal to the coverslip show distribution of vimentin at both the dorsal and ventral surfaces of migrating cells. Each plane taken from different positions relative to the leading of the migrating epithelial sheet. (F) Lateral maximum intensity projection of filopodial dynamics in an RPE cell, labeled with LifeAct-mNeonGreen. (G) Montage of filopodia formation from sub-region in f. (H) Dynamics of microtubule plus tips (+TIPs), labeled with EB3-mNeonGreen, in a confluent layer of U2OS cells, shown as a lateral maximum intensity projection. (I) Photobleaching comparison between pLSFM (N = 6) and single plane SPIM (N = 8). Mean normalized intensity shown, and error bars report the standard deviation. For each mode, camera integration time and illumination intensity remained constant. pLSFM achieves 2.7-fold faster volumetric image acquisition rate than single plane SPIM, without increasing the rate of photobleaching. All scale bars 10 μm.