Table 1. Primer sequences and PCR conditions used in this study.

DNA target	Primer set	Locus	Direction	Sequence	Annealing temperature	Reference
Vertebrate DNA	Kitano 12S*	12S	F	5′-CCC AAA CTG GGA TTA GAT ACC C-3′	57°	Alcaide et al. (2009)
			R	5′-GTT TGC TGA AGA TGG CGG TA-3′		Alcaide et al. (2009)
	Melton 12S	12S	F	5′-ACT GGG ATT AGA TAC CCC ACT ATG-3′	53°	Hwang et al. (2010)
			R	5′-ATC GAT TAT AGA ACA GGC TCC TC-3′		Hwang et al. (2010)
	Vert COI	COI	M13BC-FW	5′-TGT AAA ACG ACG GCC AGT HAA YCA YAA RGA YAT YGG NAC-3′	45°	Kitano et al. (2007)
			BCV-RV1	5′-GCY CAN AYY ATN CYY RTR TA-3′		Kitano et al. (2007)
	DC-CytB	CytB	UP	5-CRT GAG GMC AAA TAT CHT TYT-3	42.5°	Maslov et al. (1996)
			DW	5-ART ATC ATT CWG GTT TAA TRT-3		Maslov et al. (1996)
	Avian CytB	CytB	F	5′-GAC TGT GAC AAA ATC CCN TTC CA-3′	55°	Melton & Holland (2007)
			R	5′-GGT CTT CAT CTY HGG YTT ACA AGA C-3′		Melton & Holland (2007)
	Mammalian CytB	CytB	F	5′-CGA AGC TTG ATA TGA AAA ACC ATC GTT G-3′	55°	Melton & Holland (2007)
			R	5′-TGT AGT TRT CWG GGT CHC CTA-3′		Melton & Holland (2007)
	Vert CytB	CytB	CB1-L	5′-CCC CTC AGA ATA TTT GTC CTC A-3′	57°	Mota et al. (2007)
			CB2-H	5′-CAT CCA ACA TCT CAG CAT GAT GAA A-3′		Mota et al. (2007)
Trypanosome DNA	Tcz*	18S	18sf	5′-TTA ACG GGA ATA TCC TCA GC-3′	50°	Ngo & Kramer (2003)
			S829r	5′-GCA TCA CAG ACC TGC TGT TG-3′		Palumbi et al. (1991)