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. 2017 Aug 28;7:384. doi: 10.3389/fcimb.2017.00384

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Copyright © 2017 Kubori, Bui, Hubber and Nagai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Legionella interferes with the recruitment of ubiquitin-dependent adaptor proteins to SCVs in a T4SS- and RavZ-dependent manner. HeLa cells expressing GFP-p62, GFP-NDP52, or Tecpr1-GFP were co-infected with Salmonella expressing mCherry and Legionella for 1 h. (A) Recruitment of the adaptor proteins to SCVs was visualized by fluorescence microscopy. Adaptor-positive SCVs are indicated by arrows. Average percentage of p62 (B), NDP52 (C), or Tecpr1 (D)-positive SCVs in transfected cells was calculated from three independent examinations. Data present the average ± SD; ^*P < 0.02, ^**P < 0.01.