Table 2.
Proteins present in human blood eosinophil (EOS) and involved in the phosphoinositide 3-kinase/mammalian target of rapamycin pathway.
| Protein/gene name | Protein expression (iBAQ/10000) | Phosphorylated sites |
|---|---|---|
| AKT1 (PKB) | 6656 | Not detected |
| AKT2 (PKB) | 2282 | S478 |
| FKBP1A (FKBP12) | 1997100 | Not detected |
| GSK3A | 9069 | Not detected |
| GSK3B | 12297 | S9 |
| INPP5D (SHIP) | 53856 | S243, S971, S1039 |
| MLST8 | 3323 | Not detected |
| MTOR | 1503 | Not detected |
| PDCD4 | 41522 | T90, S94 |
| PDPK1 | 21325 | Not detected |
| PIK3CA | 94 | Not detected |
| PIK3CB | 2190 | Not detected |
| PIK3CD | 8867 | Not detected |
| PIK3CG | 9815 | Not detected |
| PTEN | 9587 | Not detected |
| RHEB | 10969 | Not detected |
| RICTOR | 1251 | Not detected |
| RPS6 | 143160 | S235, S326 |
| RPS6KB1 (p70S6K) | 970 | Not detected |
| RPS6KB2 (p70S6Kb) | 1869 | Not detected |
| RPTOR | 710 | S863 |
| TSC1 | 3037 | Not detected |
| TSC2 | 2707 | S1420 |
75 million EOS from 3 different donors were analyzed using two-dimensional liquid chromatography coupled with high-resolution mass spectrometry to generate a proteome and phospho-proteome in resting EOS (3). Intensity-based absolute quantification (iBAQ) and phosphosites are shown.