Table 3.
Proteins present in human eosinophil and involved in the mitogen-activated protein kinase signaling upstream of protein translation.
| Protein/gene name | Protein expression (iBAQ/10000) | Phosphorylated sites |
|---|---|---|
| MAPK3 (ERK1) | 57843 | Not detected |
| MAPK1 (ERK2) | 119320 | Not detected |
| MAPK14 (p38α) | 22843 | Not detected |
| MAPK11 (p38β) | Not detected | Not detected |
| RPS6KA1 (p90S6K, RSK1) | 75911 | T393, S389, S372 |
| RPS6KA2 (p90S6K, RSK3) | 21015 | T595, S402 |
| RPS6KA3 (p90S6K, RSK2) | 26924 | T577, S227, S386, T231, S369 |
| MKNK1 (Mnk1) | 3601 | S221 |
| MKNK2 (Mnk2) | 112 | Not detected |
| MAPKAPK2 (MK2) | 44194 | Not detected |
| MAP2K2 (MEK2, upstream ERK) | 153200 | S226, T394 |
| MAP2K4 (MEK4, upstream p38) | 14813 | S91, T89 |
| MAP3K3 (MEKK3, upstream ERK) | 5375 | S178, S270, S281 |
75 million EOS from 3 different donors were analyzed using two-dimensional liquid chromatography coupled with high-resolution mass spectrometry to generate a proteome and phospho-proteome in resting EOS (3). Intensity-based absolute quantification (iBAQ) and phosphosites are shown.