Table 1.

Enrichment and recovery of small molecule-DNA conjugates from sulfonyl fluoride (3)-based crosslinking or solid-phase affinity selections for ligands binding to B. CAII. Enrichment is given as the fold change in concentration of each ligand-DNA construct relative to the non-ligand (Bz) DNA construct. Recovery is given as the amount of DNA recovered of the initial DNA mixture.

	SDS + Stringent Washes		Solid-Phase Affinity Pulldown
	X-linking with 3 1 μM CAII Enrichment (Recovery)	Without X-linking 1 μM CAII Enrichment (Recovery)	1 μM CAII Enrichment (Recovery)	17 μM CAII Enrichment (Recovery)
GL-CBS (Kd 120 nM)	17,000 (11 %)	22 (0.021 %)	720 (52 %)	610 (89 %)
D-CBS (Kd 4.5 μM)	1,700 (1.1 %)	28 (0.026 %)	2.2 (0.16 %)	25 (3.6 %)
Bz (non-ligand)	(0.00062 %)	(0.00094 %)	(0.073 %)	(0.15 %)