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. Author manuscript; available in PMC: 2018 Sep 7.
Published in final edited form as: Mol Cell. 2017 Aug 31;67(5):891–898.e4. doi: 10.1016/j.molcel.2017.08.002

Figure 3. MRN nucleolytically removes Ku-blocked DNA ends.

Figure 3

(A) Illustration and (B) kymographs of MRN co-localizing with Ku. Two MRN complexes initially bind ~25 kb and ~10 kb upstream of Ku but rapidly slide to the DNA end (top). White arrows indicate binding events. In the presence of Mn2+ and ATP, MRN nucleolytically removes itself and Ku (bottom, red arrows). (C) Lifetime of Ku on DNA ends in the presence of nuclease active MRN sub-complexes, or the nuclease-deficient M(H129N)RN. (D) Half-lives with two additional controls added in black.