| Antibodies |
| Monoclonal ANTI-FLAG M2 antibody produced in mouse |
Sigma-Aldrich |
F1804-1MG |
| HA tag antibody (mouse) |
GeneTex |
GTX18181 |
| Anti-Mouse IgG (H&L) IRDye800 Conjugated Antibody |
Rockland |
RL-610-132-121 |
| Rabbit anti-HA Tag antibody affinity purified |
ICL Lab |
RHGT-45A-Z |
| Monoclonal ANTI-FLAG BioM2 antibody produced in mouse |
Sigma-Aldrich |
F9291-.2MG |
| F(ab′)2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, Qdot 705 |
Life Tech. |
Q11461MP |
| F(ab′)2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, Qdot 605 |
Life Tech. |
Q11402MP |
|
|
|
| Bacterial and Insect Strains |
| BL21(DE3) cells |
Agilent |
230245 |
| ArcticExpress (DE3)RIL cells |
Stratagene |
230193 |
| DH10bac cells |
Invitrogen |
10361012 |
| Sf21 insect cells (Grace’s media) |
Invitrogen |
B82101 |
|
|
|
| Chemicals, Peptides, and Recombinant Proteins |
| Streptavidin Atto647N |
Atto-Tec |
AD 647N-65 |
| Streptavidin Quantum Dot 705 |
Life Tech. |
Q10163MP |
| Streptavidin Quantum Dot 605 |
Life Tech. |
Q10103MP |
| Dynabeads M-280 Streptavidin |
Life Tech. |
11205D |
| T4 Polynucleotide Kinase |
NEB |
M0201L |
| [γ-32P]-ATP |
Perkin Elmer |
BLU002H |
| Bovine Serum Albumin (BSA) |
Fisher Scientific |
9048-46-8 |
| DNase I |
NEB |
M0303S |
|
|
|
| Critical Commercial Assays |
| Q5 PCR Mutagenesis |
NEB |
E0554S |
| Bac-to-Bac Baculovirus Expression System |
Life Tech. |
|
|
|
|
| Deposited Data |
| Mendeley Data |
Mendeley |
doi:10.17632/4ck8jnr2mx.1 |
| Oligonucleotides |
| See Table S2 for a list of oligonucleotides used in this study |
IDT |
|
| Recombinant DNA |
| pFastbac1 |
Life Tech. |
10360014 |
| Lambda DNA |
NEB |
N3011S |
| pUC19 vector |
NEB |
N3041S |
| TOPO blunt vector |
Stratagene |
240205 |
| Ku70-His (pDR121), Ku80-3XHA (pIF2) |
This study |
Available upon request |
| Human Mre11-3XFlag (pIF240), Rad50-His (pTP3090), Nbs1 (pTP284), Mre11(H129N)-3XFlag (pTP3695), Nbs1-Flag (pTP288) |
This study |
Available upon request |
| Rad50(ΔCC)-HA-His (pTP516) |
This study |
Available upon request |
| Yeast Mre11-His (pTP391), Rad50-Flag (pTP3335), Xrs2 (pTP622) |
This study |
Available upon request |
| gp46 (pIF237), gp47 (pIF238) |
This study |
Available upon request |
| Exo1-avidity (pIF7), Exo1(NTD)-avidity (pIF241) |
This study |
Available upon request |
| RPA-His (pIF47), RPA-GFP-His (pIF48) |
This study |
Available upon request |
| Human histone 3XHA-H2A (pIF131), H2B (pIF10), H3 (pIF11), H4 (pIF12) |
This study |
Available upon request |
|
|
|
| Software and Algorithms |
| FIJI (Fiji is just imageJ; version:1.47b) |
ImageJ software |
|
| Matlab (version:R2015b) |
Mathworks |
|
| Custom FIJI and Matlab scripts |
This study |
Available upon request |
| Other |
| Eclipse Ti-E Inverted TIRF Microscope |
Nikon |
MEA53100 |
| 60X water-immersion objective (1.2NA) |
Nikon |
MRD07602 |
| 500 nm long-pass filter |
Chroma |
ET500lp |
| 638 nm dichroic beam splitter |
Chroma |
Custom Order |
| iXon X3 DU897 EMCCD cameras |
Andor |
77026031 |
| Protein Lo-Bind Tubes |
Eppendorf |
022431081 |
| Typhoon FLA 9500 |
GE Healthcare |
28996943 |
| Optima XE ultracentrifuge |
Beckman-Coulter |
A99836 |
| Sonic Dismembrator Model 705 |
Fisher Scientific |
FB705110 |
| Ni-NTA Superflow Resin |
Qiagen |
30410 |
| HiTrap Q HP column |
GE Healthcare |
17-1153-01 |
| Anti-FLAG M2 Affinity Gel |
Sigma Aldrich |
A2220 |
| Chitin Resin |
NEB |
S6651S |
| HiTrap SP HP column |
GE Healthcare |
17115101 |
| HiTrap Heparin HP column |
GE Healthcare |
17040601 |
| Superose 6 (10/300) |
GE Healthcare |
17517201 |
| Superdex 200 (10/300) |
GE Healthcare |
17517501 |
| Softlink Soft Release Avidin Resin |
Promega |
V201A |
