Table 1. Thermodynamic parameters (and respectively standard deviations) of HA-tagged VCA nanobodies interacting with synthetic VCA peptide, determined by ITC.
| Parameters | VCA Nb2 | VCA Nb7 | VCA Nb13 | VCA Nb14 |
|---|---|---|---|---|
| N# | 0.912 ± 0.037 | 0.905 ± 0.037 | 0.757 ± 0.035 | 0.780 ± 0.031 |
| ΔH (cal/mol) | -4657 ± 254 | -4686 ± 258 | -6967 ± 433 | -3194 ± 165 |
| ΔS (cal/mol) | 12.8 | 12.4 | 5.2 | 16.0 |
| ΔG (cal/mol)* | -8537.45 | -8445.18 | -8551.53 | -8044.56 |
| Kd (μM) | 0.699 ± 0.154 | 0.820 ± 0.201 | 0.685 ± 0.133 | 1.597 ± 0.249 |
#Molar ratio or stoichiometry
*ΔG = ΔH−T * ΔS with T = 303.15 K