Fig 4. Expression of BcFLA1 as affected by CRISPR/Cas9-induced gene editing and complementation, respectively.

Total BcFLA1 expression (A), expression of BcFLA1a_mut (B) and expression of the wildtype BcFLA1 gene (C). Each bar represents the expression in one single transgenic root measured by three technical replicates with the horizontal line representing the corresponding mean of all biological replicates. Numbers indicate the respective transgenic roots. Fold over reverence = 2^{-delta Ct}; n.d. = not detected.