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. 2017 Sep 18;12:6937–6947. doi: 10.2147/IJN.S141407

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© 2017 Tang et al. This work is published and licensed by Dove Medical Press Limited

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Time-dependent uptake of FPA/EPI NPs by KCs.

Notes: KCs were incubated with different endocytosis inhibitors for 2, 6, or 18 h prior to addition of 200 µg/mL FPA/EPI NPs, and the uptake percentage was quantified by fluorometry compared with untreated controls. Data are represented as mean ± SD of five determinations. ^*P<0.05, ^**P<0.01 compared to respective controls.

Abbreviations: AMR, amiloride; Col, colchicine; CPZ, chlorpromazine; FPA/EPI NPs, epirubicin-loaded folic acid-conjugated pullulan acetate nanoparticles; KCs, Kupffer cells; NY, nystatin; PDTC, pyrrolidine dithiocarbamate.