Figure 5. Multiplexed miRNA assay.
(a) Schematic of decoding of shape-encoded microparticles. Particles used for detection of each target miRNA have distinct codes. (b) Multiplexed miRNA assays for three distinctive dysregulation patterns. + and – signs represent 1 amol of spiked-in target and the negative control, respectively. (c) Bright field and fluorescent images of a microfluidic channel for the dysregulation pattern presented in the first row of figure 4b (let7a: +, miR145: +, miR221: -). Scale bars are 50 (b) and 200 µm (c).