Skip to main content
. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: J Immunol. 2017 May 24;199(1):278–291. doi: 10.4049/jimmunol.1700339

Figure 1.

Figure 1

TLR-stimulation induces complement gene upregulation via autocrine C3ar1/C5ar1 signaling in DCs. A. Purified OTII CD4+ cells (1x106) mixed with splenic CD11c+ DCs (2.5x105) were tested in IFNγ ELISPOT assays upon stimulation with varying concentrations of ova323-339 (left panel, arrow indicates lowest concentration of antigen to initiate a response) or various PAMPs with (black) without (white) antigen (right 3 panels, arrows indicate sub-threshold levels of each PAMP that did not induce IFNγ production). *p<0.05 compared to baseline, bars represent means and s.d. of triplicates. Each experiment was repeated at least once. B. Relative C3, fB and fD gene expression (RT-PCR) of cultures containing OT-II cells plus splenic APCs from WT or various complement component deficient animals as indicated ± 0.1 μg/ml ova323-339 ± 0.1 μg/ml pI:C or 0.1 μg/ml LPS or 0.1 μg/ml CpG for 1 h. Each bar shows mean and s.d. of triplicate values and is representative of at least 2 individual experiments. *p<0.05 compared to unstimulated baseline. C–D. Relative expression (qRT-PCR) of C3 (left) and fD (right) in RNA obtained from OTII cells mixed with DCs from WT, Ticam1−/− (C) or MyD88−/− (D) mice as indicated ± 0.1 μg/ml ova323-339 ± 0.1 μg/ml Poly I:C or 0.1 μg/ml LPS. *p<0.05. Each bar shows mean + s.d. of triplicate values and is representative of 3 individual experiments.