Fig. 8. AIE leads to decreased ChAT+/ nestin+ cell populations in the Nucleus basalis of Meynert (NbM).
(A) Estimated total number of ChAT+/ nestin− and ChAT+/ nestin+ cells in the NbM. AIE significantly decreased the ChAT+/ nestin+ cell population in the NbM (T; p<0.05). There was a trend for AIE to decrease the estimated ChAT+/ nestin− cell population (t; p=0.08). (B) Number of cells (per mm2) in the NbM. AIE leads to a significant reduction in the total number of cholinergic cells in the NbM (T; p<0.05), and a significant reduction in the number of ChAT+/ nestin+ cells (T; p<0.05). There was a trend in the reduction of ChAT+/ nestin− cells per mm2 as a result of AIE (t; p=.06). (C) Average soma area of ChAT+/ nestin− and ChAT+/ nestin+ cells in the NbM. AIE did not +significantly affect soma area in either cell population.